Acid precipitation of proteins for lipid peroxidation assay

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hanhan2008
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Acid precipitation of proteins for lipid peroxidation assay

Post by hanhan2008 » Wed Dec 05, 2007 3:20 pm

I noticed that different researchers use different acids (TCA, perchloric acid, etc.). Do these different acids make a difference? Could these acids also precipitate some other things (non-protein staff)? Could perchloric acid oxidize additional lipids during the assay and so make the assay unreliable? Sorry for my poor background in macromolecular chemistry. Thanks.

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