making a graph of step ladder dna electrophoresed thru...

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Trishntonia
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making a graph of step ladder dna electrophoresed thru...

Post by Trishntonia » Tue Dec 04, 2007 5:00 am

...Agarose Gel
That title alone will stray ppl away from bio...: )

I have to make a graph, but I am just not understanding!

The directions say measure the distance each band traveled from the bottom of the well to the bottom of the band. Convert the base paiirs into their logs...

What am I supposed to measure it in? Inches, centimeters....and then how do you convert the base pairs into their logs? If I can get past these 2 things, I'm sure I can do the rest.

Attached is the pic my prof sent me.

Please help quick!
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2007-09-20_D1S80crt.jpg

blcr11
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Post by blcr11 » Tue Dec 04, 2007 10:43 am

Measure the distance in whatever units are most convenient, doesn't matter which. Then you should know the number of base pairs in each band of the ladder. This could be anything from 50 to tens of thousands of base pairs depending on the specific ladder you're using. Take the log of each length of base pairs. For example, if the band has 1x10^3 bp, the log is 3. Plot distance moved on the x-axis and log(bp) on the y-axis and you should get a decent straight line. To estimate the length of an unknown, measure the distance the unknown moved, find the log(bp) this corresponds to, take the antilog of that number and you have an estimate for the unknown length in bp.

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