Tris-Cl buffer and temperature

Debate and discussion of any biological questions not pertaining to a particular topic.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
User avatar
redondoself
Garter
Garter
Posts: 4
Joined: Fri Apr 14, 2006 5:50 am
Location: Beijing

Tris-Cl buffer and temperature

Post by redondoself » Fri Nov 30, 2007 8:48 am

Hi~ everyone!

We know that the pH value of Tris-Cl buffer often changes when the temperature is different. Usually, we adjust the pH value at room temperature(25℃). But, my buffer always used at 4 ℃.Then, my question is at which temperature should I fix the final pH value?

Thanks a lot!
God help one who helps himself!


[email protected]

blcr11
Viper
Viper
Posts: 672
Joined: Fri Mar 30, 2007 4:23 am

Post by blcr11 » Fri Nov 30, 2007 9:25 am

Usually you make your Tris buffer at room temp for a 25C pH that will temperature-adjust to the pH you want at 4-5C. The rule of thumb is that, between 25C and 5C, the pH of a Tris buffer will increase by 0.03 pH units per degree fall in temperature. So, if you want pH 7.6 at 4C, prepare the buffer at pH 7 at room temperature. If you need pH 7 or so at 4C, you might want to use another buffer as Tris won't behave well at 4C for that pH--that is, it won't be a very good buffer. There are tables for more exact temperature compensations. Here is one: http://www.neb.com/nebecomm/tech_refere ... buffer.asp

User avatar
redondoself
Garter
Garter
Posts: 4
Joined: Fri Apr 14, 2006 5:50 am
Location: Beijing

Post by redondoself » Mon Dec 03, 2007 4:11 pm

Thank you a lot! :D
God help one who helps himself!


[email protected]

Post Reply

Who is online

Users browsing this forum: No registered users and 5 guests