glass vs. plastic when working with lipids

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unibas
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glass vs. plastic when working with lipids

Post by unibas » Wed Nov 28, 2007 8:59 pm

Hi,

I need to analyze the fat content of stool samples from mice. I found a protocol that involves chloroform:methanol extraction and weighing the extracted fat.
The entire protocol is with glass tubes, but we don't have a centrifuge that fits glass tubes. How important is it to use glass and not plastic? I anyway can't use glass, so is it worth bothering with this protocol?

Thanks!

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MrMistery
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Post by MrMistery » Wed Nov 28, 2007 9:22 pm

i don't see how glass/plastic tubes would make a significant difference. But my lab experience is extremely limited.
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unibas
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Post by unibas » Wed Nov 28, 2007 9:27 pm

I suppose lipids stick to plastic. I think most of the protocols for lipid analysis ask for glass tubes.

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Post by MrMistery » Wed Nov 28, 2007 9:30 pm

they do stick to plastic, but if they are in a methanol chloroform solution i doubt they do
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