Extraction of mRNA

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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micolo123
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Extraction of mRNA

Post by micolo123 » Wed Nov 21, 2007 7:43 pm

What is a convenient way to 'fish out' messenger RNA from a pool of total RNA (also containing rRNA and tRNA)?

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canalon
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Post by canalon » Thu Nov 22, 2007 1:31 am

In bacteria? It's just a pain. AFAIK the are no easy ways like in eukaryotes :( There are a few kits that pretends to be able to improve things (I think epicentre has one) but even then you just enrich in mRNA
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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