Enzyme graphs

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dan167
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Enzyme graphs

Post by dan167 » Tue Nov 20, 2007 10:09 pm

Which is more accurate graph for measuring V-max and Km. Michaelis, Hands or lineweaver-burkes? I guess michaelis is the least as the V-max is never really reached. But I am not sure about the rest.

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MrMistery
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Post by MrMistery » Wed Nov 21, 2007 7:35 am

no idea what Hands is, but LB is very simple and is probably the answer
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dan167
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Post by dan167 » Wed Nov 21, 2007 9:29 am

How do you work out the v-max from the LB?

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Post by blcr11 » Wed Nov 21, 2007 12:34 pm

For Lineweaver-Burke plots the x-intercept is -1/Km, the y-intercept is 1/Vmax, and the slope is Km/Vmax. You can also do Eadie-Hofstee plots (v = -Km(v/[S]) + Vmax) in which case the x-intercept is Vmax/Km, the y-intercept is Vmax, and the slope is –Km. Lineweaver-Burke plots are more commonly used, though the Eadie-Hofstee plots sometimes give more accurate estimates of the parameters.

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