centrifugation

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gumshoes_15
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centrifugation

Post by gumshoes_15 » Mon Nov 12, 2007 10:25 pm

What are the disadvantages and advantages of using centrifugation to extract lipids?

Promethean
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Post by Promethean » Fri Nov 23, 2007 4:03 am

Can you expand on that? A little more detail on what you are trying do would be helpfull?

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MrMistery
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Post by MrMistery » Fri Nov 23, 2007 12:55 pm

i've never heard of extracting lipids with a centrifuge. usually it's done with a solvent that disolves lipids but not anything hydrophilic. I don't see why you would bother using a centrifuge.
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biohazard
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Post by biohazard » Sat Nov 24, 2007 9:04 pm

Maybe this is about separating cell organelles (including any lipid-containing ones) with a gradient (ultra)centrifugation? At least that method is fairly straightforward to do if you have the equipment. I'm not sure either if I've heard of any other lipid separation protocols using centrifuges :P

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