Identifying Protiens

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Ciwan
Garter
Garter
Posts: 25
Joined: Fri Sep 21, 2007 10:11 pm

Identifying Protiens

Post by Ciwan » Fri Nov 09, 2007 8:48 am

Hello guys

can someone explain to me how we can identify wherether a protien has a secondary or a globular structure ?

Thanks

blcr11
Viper
Viper
Posts: 672
Joined: Fri Mar 30, 2007 4:23 am

Post by blcr11 » Fri Nov 09, 2007 1:05 pm

Almost all proteins have secondary structure of some kind--even globular proteins. If you are asking how to tell what kind of structures are there, then you are talking about things like ORD-CD (tells you something about the relative amount of helix vs strands), spectroscopy (noes from nmr tells you something about helices and strands), centrifugation (tells you something about the overall shape). You can always attempt to crystallize a protein and determine its x-ray structure. That way you can actually "see" the secondary, tertiary, and sometimes quaternary structures of a protein or protein complex.

pluto
Garter
Garter
Posts: 6
Joined: Thu Nov 15, 2007 6:46 am

Post by pluto » Thu Nov 15, 2007 6:52 am

another way to do it is in silicia with prediction software

Post Reply

Who is online

Users browsing this forum: No registered users and 6 guests