problems growing plasmid

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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ambarrie
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problems growing plasmid

Post by ambarrie » Tue Nov 06, 2007 5:44 pm

Hi all,

I've been trying to grow up e. coli on an agar plate, and I keep getting small colonies that are very close together. I pipetted 100ul of my transformation onto the plate and then streaked it into 4 quadrants. Are colonies that are the size of approx. a pin head and close together okay? It was difficult to innoculate, but I just wanted to make sure this will work. I did it last week, and my DNA yield after purification was somewhat low. Thanks!

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canalon
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Post by canalon » Wed Nov 07, 2007 1:54 am

Sounds Ok to me after overnight growth on LB supplemented with anibiotic.
Although after transformations I usually do not streak, but rather spread to get a good separation of colonies
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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