Question about Conjugation. Kind of urgent.

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az_0k
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Question about Conjugation. Kind of urgent.

Post by az_0k » Sun Nov 04, 2007 12:10 am

Hi all.
I just have a little question regarding conjugation. I did my first conjugation experiment last tuesaday between the donor E. coli pKM101 with ampicilin resistance plasmid and the plasmid-fre recipient E. coli WL400 strain. I noticed that the two cultures were washed in saline solution (0.85% NaCl) prior to conjugation. Could someone please tell me the significance of this washing step? Thank you very much

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canalon
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Post by canalon » Sun Nov 04, 2007 12:23 am

To wash everything that could inhibit the conjugation...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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