Identfying Bacteria From Pictures

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Cfoster
Garter
Garter
Posts: 6
Joined: Fri Nov 02, 2007 8:15 pm

Identfying Bacteria From Pictures

Post by Cfoster » Fri Nov 02, 2007 9:09 pm

Need some help people. Can somebody please Help me, there are some really cool samples, but I just don't have enough knowledge of what some of the bacteria species are. I can email all of the pics to whomever is interested in helping our local Health Department.

Please comment and ask questions if you wish!
Attachments
excersise ball.JPG
s changeroom bench1.JPG

User avatar
victor
King Cobra
King Cobra
Posts: 2668
Joined: Sat Apr 30, 2005 12:01 pm
Location: Yogyakarta, Indonesia..
Contact:

Post by victor » Sat Nov 03, 2007 5:40 am

I think you can't determine the genus of these bacteria if you only have these two photos. At least you have to do several cultural, staining and biochemical tests to ensure which genus do these two bacteria belong...
have you provide the characterization processes on these two?
Q: Why are chemists great for solving problems?
A: They have all the solutions.

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Sat Nov 03, 2007 5:32 pm

Knowing the kind of plate would definitely help.

But even selective media usually require confirmation after selection. If I assume that the top picture is from a plate of mFC-BCIG incubated at 44.5°C I would say that the blue colonies are likely to be E. coli. You could confirm that by checking for lactose fermentation and production of indole for example.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

User avatar
victor
King Cobra
King Cobra
Posts: 2668
Joined: Sat Apr 30, 2005 12:01 pm
Location: Yogyakarta, Indonesia..
Contact:

Post by victor » Sun Nov 04, 2007 2:31 am

wow, you are a plate master, Canalon :wow:
Q: Why are chemists great for solving problems?
A: They have all the solutions.

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Mon Nov 05, 2007 1:16 am

No I am simply a microbiologist...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Cfoster
Garter
Garter
Posts: 6
Joined: Fri Nov 02, 2007 8:15 pm

Post by Cfoster » Mon Nov 05, 2007 8:18 pm

canalon wrote:Knowing the kind of plate would definitely help.

But even selective media usually require confirmation after selection. If I assume that the top picture is from a plate of mFC-BCIG incubated at 44.5°C I would say that the blue colonies are likely to be E. coli. You could confirm that by checking for lactose fermentation and production of indole for example.


The clear slide is ChromAgar if that helps.

Cfoster
Garter
Garter
Posts: 6
Joined: Fri Nov 02, 2007 8:15 pm

Post by Cfoster » Mon Nov 05, 2007 8:31 pm

I do have more slides if that will help!

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Tue Nov 06, 2007 12:13 am

Chromagar is a brand, they do multiple plates. I can see that it is their orientation medium so you will be able to identify the different colonies with that:
http://www.chromagar.com/products/orientation.html
Honestly with those kind of products that is all i can say.

Another thing. If ypu want to send more slide, each slide should tell what kind of plate it was. And there are quite a lot of colonies meaning that there is a lot of mixed bacteria in your sample, so you have to give an idea of what you plated too for any kind of help.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Cfoster
Garter
Garter
Posts: 6
Joined: Fri Nov 02, 2007 8:15 pm

More Slides

Post by Cfoster » Tue Nov 06, 2007 5:35 am

All the information I have is this:

the clear slides are ChromAgar plates
the red ones are BloodAgar (sheeps blood)

I hope that helps but understand if it's not enough. I can get more specfic answers if I had the right questions as well.

thank you all for your help, appreciate it
Attachments
excersise ball.JPG
s changeroom bench1.JPG
s shower floor.JPG

Cfoster
Garter
Garter
Posts: 6
Joined: Fri Nov 02, 2007 8:15 pm

Post by Cfoster » Tue Nov 06, 2007 5:38 am

First Slide is of a mens shower room floor

Second one is Mens workout bench

Third one is an excercise ball

Hope that helps people!

thanks, all hypothesies are welcome here. I need to know what tests to pay for!

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Wed Nov 07, 2007 1:52 am

The link I gave you should help you identify on the Chromagar orientation plate. The 2 other includes some beta hemolytic colonies, likely S. aureus but that is all I can say.

As for asking what kind of test to ask, it depnds on what is your problem. Culturing bacteria is nice but there tons of the litle things everywhere and some are likely to be pathogenic. But if you have a specific problem then you should define the best strategy to solve it a priori, rather than a posteriori, because you will find plenty of bacteria but you will not be able to know which one are interesting.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Cfoster
Garter
Garter
Posts: 6
Joined: Fri Nov 02, 2007 8:15 pm

Thanks

Post by Cfoster » Tue Nov 27, 2007 3:38 pm

Thanks for the help all!

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest