How specific is an ELISA plate in binding proteins?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
hanhan2008
Garter
Garter
Posts: 26
Joined: Wed Sep 05, 2007 5:41 pm

How specific is an ELISA plate in binding proteins?

Post by hanhan2008 » Fri Oct 19, 2007 6:16 pm

Can these plates really only bind proteins, not lipids or other things in cell lysate? How?
Thanks, Hanhan

weesper
Death Adder
Death Adder
Posts: 82
Joined: Sun May 29, 2005 1:57 pm

Post by weesper » Sun Oct 21, 2007 9:46 am

well antibodies can of course be raised against DNA, lipidmolecules etc as well and in that sense it all depends on which antibodies your elisa plate was coated with in other words which is specificity is. in any experiment there are false positives (ie binders that are not those of the predicted specificity) but you diminish with frequent washes of the plate and usually non-specific binders are of a lower affinity and washed away even all the other stuff thats in your lysate

Post Reply

Who is online

Users browsing this forum: No registered users and 4 guests