thermus aquaticus

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderators: Leonid, amiradm, BioTeam

aztectgirl
Garter
Garter
Posts: 4
Joined: Mon Oct 08, 2007 11:42 pm

thermus aquaticus

Post by aztectgirl » Mon Oct 08, 2007 11:45 pm

Ok.. so how do the enzymes of this bacteria compare with other microorganisms' enzymes?? I know they are function at high temp and reproduce very fast! but what else?? How might they prove to be valuable in the future? Any help appreciated. I have been searching online forever.

User avatar
mith
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN
Contact:

Post by mith » Mon Oct 08, 2007 11:50 pm

pcr
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

BDeis
Garter
Garter
Posts: 11
Joined: Mon Aug 20, 2007 2:46 am

Post by BDeis » Tue Oct 09, 2007 4:54 pm

As mentioned above, the dna polymerase of this org. is used in pcr. Hence, the Taq bead. Since they live in high temps the polymerase can handle the high denaturing temps of pcr. If you were using mesophilic proteins they would denature during each cycle. This would leave you a big gooey mess and no dna amplification. That is the current use. As for future uses I don't know. Perhaps you can expand on future uses of pcr technology.

User avatar
mith
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN
Contact:

Post by mith » Tue Oct 09, 2007 5:14 pm

I just learnt this today, pcr was invented by a surfer dude. Rad!
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Wed Oct 10, 2007 12:21 pm

karry mullis or the guy he stole the idea from?
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

User avatar
victor
King Cobra
King Cobra
Posts: 2668
Joined: Sat Apr 30, 2005 12:01 pm
Location: Yogyakarta, Indonesia..
Contact:

Post by victor » Sat Oct 20, 2007 5:30 am

Karry Mullis is a surfer? wow....
I also wondering, what force that makes Taq polymerase can withstand high temperature? how can it's 3D structure can does that....
Q: Why are chemists great for solving problems?
A: They have all the solutions.

Jammerz
Death Adder
Death Adder
Posts: 77
Joined: Thu Mar 22, 2007 4:02 am
Location: USA

Post by Jammerz » Sat Oct 20, 2007 7:06 am

Probably a large number of S-S disulfide bonds in addition to much hydrogen bonding. But I'm only guessing, don't quote me on that.

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Sat Oct 20, 2007 7:33 am

I was gonna make exact the same guess :lol:
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

User avatar
victor
King Cobra
King Cobra
Posts: 2668
Joined: Sat Apr 30, 2005 12:01 pm
Location: Yogyakarta, Indonesia..
Contact:

Post by victor » Mon Oct 22, 2007 9:27 am

thanks for the informations boss... :D
hmm....there must be another else that makes this thing soooo strong :-?
I really curious about those force....
Q: Why are chemists great for solving problems?
A: They have all the solutions.

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Mon Oct 22, 2007 8:20 pm

http://www.rcsb.org/pdb/explore.do?structureId=1TAQ
Here, download the structure and explore it. I do not have Chimera on this computer, but you should be able to deduce by analysing it with Chimera what are the main bonds.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

User avatar
mith
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN
Contact:

Post by mith » Mon Oct 22, 2007 8:40 pm

chimera is free :)
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Tue Oct 23, 2007 9:45 am

i know, but this is my sister's laptop and she has a strict "No biochemistry geekish stuff on my laptop" rule
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

Post Reply

Who is online

Users browsing this forum: No registered users and 0 guests