yeast-2-hybrid

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
hussainmb
Garter
Garter
Posts: 1
Joined: Mon Sep 17, 2007 8:37 pm

yeast-2-hybrid

Post by hussainmb » Mon Sep 17, 2007 8:43 pm

Hi All,

i have encountered a rather strange problem.

I recently performed the yeast-2-hybrid experiment with a cDNA library to identify potential interacting partners to my target protein. After performing the experiment and plating cells on media with X-gal, i do see Blue colonies, but...

rather than growing on the agar, the colonies seem to enter the agar and grow into it. I tried to isolate plasmid from these colonies, but was not succesful.

Any clues as to why they may be growing into the agar?

Thanks,
hussain

Post Reply

Who is online

Users browsing this forum: No registered users and 5 guests