Enzyme manufacture

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genovese
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Enzyme manufacture

Post by genovese » Thu Aug 30, 2007 4:11 am

I know that some enzymes are manufactured commercially by various processes. Have we been able to manufacture an enzyme by simply stringing amino acids together? Have we been able to design a completely new, previously non-existent, enzyme?

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mith
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Post by mith » Thu Aug 30, 2007 5:25 am

Making a gene sequence is easier than making an amino acid chain
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Post by genovese » Thu Aug 30, 2007 11:04 am

Thank you for that. So are commercially made enzymes all made by recombinant gene technology?
Secondly, have we ever been able to make a previoiusly non existent enzyme?

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Post by canalon » Thu Aug 30, 2007 12:38 pm

genovese wrote:Thank you for that. So are commercially made enzymes all made by recombinant gene technology?


As far as I know, yes. It is easier and cheaper.

genovese wrote:Secondly, have we ever been able to make a previoiusly non existent enzyme?


Yes and no. New functions and fusion proteins have been created, but all starting from original mutated enzyme tha have been more or less mutated and selected for the new function. As far as I know (but I don't know everything) nobody first created an enzyme on the drawing board, then translated it from scratch in DNA and had it produced.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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Post by genovese » Thu Aug 30, 2007 1:49 pm

Thank you for your prompt answer.

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Post by MrMistery » Fri Aug 31, 2007 1:12 pm

In theory it is possible. To have a chemical reaction a design an enzyme specifically for the reaction. But the process is very complex and would constitute such an incredible feat that i am sure we would have heard about it if it had been done.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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