saving my minigel

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bluerose
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saving my minigel

Post by bluerose » Sat Aug 18, 2007 9:29 pm

Is there a way to save my minigel which I ran DNA onto already this morning, so that the bands don't diffuse over the weekend? I was told that if I left it in the TAE buffer, my DNA will diffuse out after a few hours. Is there any way to prevent this long enough and save my gel? I need it for the future. Could I wrap it up and place it in the freezer? If so, at what temperature?

Thanks

blcr11
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Post by blcr11 » Sat Aug 18, 2007 10:39 pm

Not a great idea. About the best you can do is wrap it in saran wrap and put it in the cold, but there will still be some diffusion. If you're extracting or transferring to a blot, you're better off doing it right off rather than waiting til Monday. What, hot date or something?

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