E-PCR confusion

Everything on bioinformatics, the science of information technology as applied to biological research.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
dinofernando
Garter
Garter
Posts: 14
Joined: Sat Aug 04, 2007 1:50 am
Location: Oshawa, Canada UOIT

E-PCR confusion

Post by dinofernando » Sat Aug 04, 2007 2:17 am

Hi Everyone,

I'm really confused by E-PCR. I know the database uses STS sites to orient the gene on the genetic map but have no idea how this is useful in E-PCR.

The second thing is the hits from E-PCR they show Genomic, mRNA, EST's. Whats the difference in all of them?

blcr11
Viper
Viper
Posts: 672
Joined: Fri Mar 30, 2007 4:23 am

Post by blcr11 » Sun Aug 05, 2007 11:52 am

http://www.genome.org/cgi/content/full/7/5/541

As I understand it, this is a way to match up potential genes you've identified in some way with known and mapped tagged sequences in a particular genome. The genomic sequence would refer to the DNA sequence, which may or may not (probably won't) be contiguous relative to its mRNA. The mRNA corresponds to the spliced message and will be a contiguous sequence. The EST is the original tagged sequence.

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest