Clumping of BHK21 cells with trypsin

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manuli
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Clumping of BHK21 cells with trypsin

Post by manuli » Mon Jul 30, 2007 12:05 am

I am using rodent cell line BHK21. They clump very badly when you trypsinize them. It becomes to count and seed.

I use DMEM 10 % FCS with 1X non-essential amino acids to grow the cells.
Does any one have any ideas regarding resolving the clumping problem??????

Beaker73
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Post by Beaker73 » Mon Jul 30, 2007 4:45 am

Perhaps a syringe with an 18g drawing up needle. Drawing the suspension up and down gently a few times often disperses clumps.

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