Karyotyping and Probing

Genetics as it applies to evolution, molecular biology, and medical aspects.

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aaroncjj_2020
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Karyotyping and Probing

Post by aaroncjj_2020 » Fri Jul 13, 2007 7:23 am

i have some uncertainty regarding robertsonian translocation. if i have only one cell, how am i able to do the karyotyping?

i understand that the Robertsonian Translocation is translocation between acrocentric chromosome. is there any probe that able to detect the translocation? (eg. between chro 14&21 and 16 and 21) thank you

sdekivit
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Post by sdekivit » Fri Jul 13, 2007 8:02 am

i would try a FISH experiment. Since a Robertsonian translocation is a dicentric fusion and the acentric parts fragment will be lost, you should be able to detect a Robertsonian translocation using probes for ed 14 & 21 (you'll find only 1 strand of both connected to each other)

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