heat shcok protocal

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mehdi71000
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heat shcok protocal

Post by mehdi71000 » Mon Jul 09, 2007 11:20 am

hi can any one explain the heat shock protocol please? and can I freeze ecoli?

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canalon
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Post by canalon » Mon Jul 09, 2007 6:53 pm

Yes you can freeze E. coli, in a 15% solution of sterile glycerol.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

mehdi71000
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Post by mehdi71000 » Wed Jul 11, 2007 12:33 pm

and if its not done by glycrol will it die?

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canalon
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Post by canalon » Wed Jul 11, 2007 5:07 pm

Ice crystals will form and destroy bacteria. You might not kill all of them, but the recovery rate is not going to be good, and if you keep your cells at -18ºC, the crystals will slowly grow and cause more and more damage... But you can use DMSO (di Methyl Sulfoxide) instead.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

mehdi71000
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Post by mehdi71000 » Thu Jul 12, 2007 9:28 am

thanks mate :D

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