SURE E. coli strain

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cleidsonn
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SURE E. coli strain

Post by cleidsonn » Wed Jun 27, 2007 7:23 pm

Hello!
I would like to know if someone can send me - or even if someone knows - a specific protocol to competent SURE E. coli strain. I get problems to do it!
Now, I'll try the "Inoue method for Ultra-competent cells". But I dont know if work well to this strain.
I'm trying to cloning small oligos to RNAi.
If someone can help me, I'll be very thankfull!

Cleidsonn

Ewa
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Post by Ewa » Wed Jul 04, 2007 6:34 pm

hi

what is the problem with making it competent? what 'standard' procedures have you tried already?

I always use 'PIPES-competent' protocol, works miracles :)

let me know if you want it

best,
Ewa

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