RNA extraction from FFPE

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: Leonid, amiradm, BioTeam

Post Reply
molecular newbie
Garter
Garter
Posts: 14
Joined: Tue Jun 27, 2006 7:12 pm

RNA extraction from FFPE

Post by molecular newbie » Tue Jun 19, 2007 7:49 pm

Has anyone else tried extracting RNA from Formilin fixed parafin tissue? and do you know of an optimal protocol for doing so? because the manufacturers protocol sucks!!!!

Thank you

Jim T
Garter
Garter
Posts: 1
Joined: Thu Jun 28, 2007 11:14 am

Re: RNA extraction from FFPE

Post by Jim T » Thu Jun 28, 2007 11:37 am

Have you tried Formapure from Agencourt Biosciences? This kit is designed for RNA extraction from paraffin embedded tissue for real time PCR or microarray use.

molecular newbie wrote:Has anyone else tried extracting RNA from Formilin fixed parafin tissue? and do you know of an optimal protocol for doing so? because the manufacturers protocol sucks!!!!

Thank you

Post Reply

Who is online

Users browsing this forum: Google [Bot] and 1 guest