Genetic engineering (insertation)

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mehdi71000
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Genetic engineering (insertation)

Post by mehdi71000 » Sun Jun 17, 2007 4:05 pm

Hi :D
I m very confused about how some process in genetic engineering are done.
Spatially the part witch dna in inserted in the nucleus. I know the heat shock protocol where calcium punctures the outer cell wall and dna is forced through. But I don’t understand the protocol for eukaryotic cells.
Can you help me understand the procedures please?
thanks

david23
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Post by david23 » Sun Jun 17, 2007 8:53 pm

the calcium phosphate method is cheap, but more work.
With eukaryotic cells since you have the microinjection needles, just get it under the microscope and look for the nucleus area, and inject like many many times to ensure a high yield.

mehdi71000
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Post by mehdi71000 » Sun Jun 17, 2007 9:25 pm

Its seems impossible . the needle is like 3 times smaller than the nucleus. im sure they use other methods. Like in Gene therapy etc..

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