Can this vector be used in plants?

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mehdi71000
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Can this vector be used in plants?

Post by mehdi71000 » Sat Jun 09, 2007 9:34 pm

hi all
Can this vector be used in plants? And small animals?
If no what’s the reason? And how can it be modified so it can perform in plants and small animals and insects?
Thanks
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canalon
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Post by canalon » Sun Jun 10, 2007 12:21 am

Not it is dsigned to be expressed in E. coli. It won't work in plants because:
- It is a prokaryote plasmid and thus probably do not have the required codes to initiate gene transcription
- It does have an E. coli replication origin, and thus even if it can be inserted in a plant cell it will not be replicated, so it cannot be transfered to the other generation when your plant cell grows.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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