replacing Cells in and outs

Discussion of all aspects of cellular structure, physiology and communication.

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david23
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Post by david23 » Tue Jun 05, 2007 11:41 am

breaking up like that isnt that likely, but there are could be other problems, which is why I asked you to clone a lot of it first, and when you inject try to do it with multiple cells.

Are you still obsessed with luciferase, it's not superior to GFP in glowing plants and animals. You are getting into too much trouble.

mehdi71000
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Post by mehdi71000 » Tue Jun 05, 2007 12:30 pm

Have your heard about Aequorin
From what I found. it requires only calcium to glow. I don’t think plants have that much calcium in them but what do I know.
Last edited by mehdi71000 on Tue Jun 05, 2007 9:38 pm, edited 1 time in total.

x__Dmitri
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Post by x__Dmitri » Tue Jun 05, 2007 12:42 pm

What's this about calcium? I drink 4 glass of milk a day. I'm good, my bone mass isn't going to disintegrate. And for all you soy milk drinkers... go suck a cow. Cow milk is real milk!

mehdi71000
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Post by mehdi71000 » Tue Jun 05, 2007 3:12 pm

Ok
There is an enzyme called luciferin-regenerating enzyme (LRE) this enzyme with the aid of D-cysteine regenerates luciferinoxide into Lucifer in. this reaction is common they said in fire fly.
what is D-cysteine is it an amino acid?
Any way david23 your right it’s too bloody complex here is the link
http://www.jbc.org/cgi/content/full/276/39/36508

Any ideas on Aequorin. From what I heard its not half bad

david23
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Post by david23 » Tue Jun 05, 2007 5:02 pm

D cysteine is an amino acid, could try. The diagram on that article uses multiple enzymes, more genes for you to use.

mehdi71000
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Post by mehdi71000 » Tue Jun 05, 2007 5:39 pm

thanks for your help guys wish I could repay you some how :oops:

mehdi71000
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Post by mehdi71000 » Tue Jun 05, 2007 9:39 pm

Have your heard about Aequorin?

david23
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Post by david23 » Tue Jun 05, 2007 11:10 pm

i have no idea, did u spell it right

mehdi71000
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Post by mehdi71000 » Tue Jun 05, 2007 11:26 pm

Yes it’s from a jelly fish called Aequorea victoria. It needs calcium to emit light .would it work with plants if they are fed calcium? I found some calcium containing fertilisers as well
If you like I can buy some and clone some for you swell.
Scroll down to the second segment Aequorin-based Assays


http://las.perkinelmer.com/Catalog/defa ... lAodb1LVVw

http://images.google.co.uk/images?hl=en ... a=N&tab=wi


http://en.wikipedia.org/wiki/Aequorin

GUS CAT NEO genes they glow as well. But no clue were to buy them from

david23
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Post by david23 » Tue Jun 05, 2007 11:47 pm

good idea, try that if u can

mehdi71000
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Post by mehdi71000 » Wed Jun 06, 2007 12:48 am

Can you direct me witch one the products is the actual vector. please
Thanks

http://las.perkinelmer.com/Catalog/defa ... lAodb1LVVw

mehdi71000
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Post by mehdi71000 » Wed Jun 06, 2007 12:00 pm

I found some GM trees in the internet. They are supposed to be super fast growing type. Is the gene responsible in a separate vector or you think it’s very complex and inserted into the actual DNA. I ask this because if I could extract the responsible vector and create my own fast growing plant would be interesting.

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