inserting GFP vector

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mehdi71000
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inserting GFP vector

Post by mehdi71000 » Thu May 17, 2007 6:45 pm

hi
what would happen if i insert GFP vecor into a fish egg's embryo. would every cell in the fish glow? if so how whould the fish see or sleep? would this stress the animal? is there a animal that dosn't sleep thay lays seethrough eggs?
thanks

david23
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Post by david23 » Thu May 17, 2007 7:30 pm

Is there a way to make your experiment questions into one topic, multiple topics are a big hassle.

for your question, all I can say is that since there are a lot of equipments and procedures missing in your plan, I would say it's pretty difficult. Even with the proper equipments, which is a lot of stuff, it is still difficult, especially since not only are you trying to inject the fish foreign DNA, but you also have to make sure it gets expressed. To answer your other question, the fish might be stressed, but the fishes that other people made lived a pretty long time. I suppose if you can get the fishes to mate and have baby glowing fishes, then that alone is more valuable because you will always have more glowing fishes

mehdi71000
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Post by mehdi71000 » Thu May 17, 2007 11:23 pm

Thanks ill try
What if I inject the embryo when it has spitted into 2 would one half of the fish glow and the other half won’t? if so i sopose and the fish can get a shut eye or half to be exact at night.:->
how about those GFPs any luck?
thanks

david23
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Post by david23 » Fri May 18, 2007 9:08 pm

you mean cell division? all the daughter cells of the original will have the gene, that is if the original insertion worked.

mehdi71000
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Post by mehdi71000 » Fri May 18, 2007 9:27 pm

cheers david23 your a star :D

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