how to produce more of one vector

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mehdi71000
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how to produce more of one vector

Post by mehdi71000 » Thu May 17, 2007 3:19 pm

hi guys
if i buy lets say 1mg of GFP on a vector how can i make more of it?
thanks

david23
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Post by david23 » Thu May 17, 2007 4:27 pm

The bacteria will clone it for you after you cultured it, grew it, and etc etc. Once thats done, all you need to do is just extract it, and do an electrophoresis to find out which is the GFP vector.

If you want to do that you'll have to buy an electrophoresis kit somewhere with all the buffers.

mehdi71000
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Post by mehdi71000 » Thu May 17, 2007 6:20 pm

thanks david
what if i give you some money and you make it for me. if all good i'll buy of you every other week.

david23
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Post by david23 » Thu May 17, 2007 7:22 pm

hmmm lets see, I would love to but i dont know what the proper procedures are for sending something like this overseas.

mehdi71000
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Post by mehdi71000 » Fri May 18, 2007 12:18 am

im in United kingdom
im sure they wont ask. you can write its a supplement. im sure they wont mind. We have GFP over here as well but its over the roof. and its only Deoxyribonucleic acid its like posting some one your toe clippings. maybe not toe clipping but you know what i mean. we got biotech’s over here as well you know. if you can’t were do you get yours from, I’ll buy of them. no problem, ill pay you for shipping and everything. How much are you selling it anyway? can you get diffrent colors? My email [email protected]
cheers

kathywilliams
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Post by kathywilliams » Mon Sep 24, 2007 7:27 am

The bacteria will clone it for you after you cultured it, grew it, and etc etc. Once thats done, all you need to do is just extract it, and do an electrophoresis to find out which is the GFP vector.

If you want to do that you'll have to buy an electrophoresis kit somewhere with all the buffers.

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