Synthesis of starch

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aceraf2612
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Synthesis of starch

Post by aceraf2612 » Tue May 15, 2007 6:23 pm

Hi

I need help on several things involving starch biosynthesis:

1. When leaf discs are placed in a glucose solution how are the glucose converted to starch
2. What is glucose-1-phosphate
3. What is necessary for the synthesis of starch
how do i measure the reate of starch production

Any help would be fantastic

Cheers :D

david23
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Post by david23 » Wed May 16, 2007 4:28 pm

oh man i just found a nice biochemical pathway map after search on google.

http://www.public.iastate.edu/~pkeeling/Pathway.htm
It even has the enzymes involved, what more can you ask.

glucose-1-phosphate is simply glucose with a phosphate group on its carbon #1 instead of the OH, typically where phosphorylation occur.

And without even looking at that website, if you want to measure rate, I suggest a nice kinetics assay, for every enzyme in the pathway.

Now if you want more, please be more specific in your questions.

aceraf2612
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Post by aceraf2612 » Thu May 17, 2007 5:36 am

what are the key variables needed for starch synthesis if the plant was given minimum resources?

Also where is AGPase located in the leaf cells?

Thanks for the help :)

david23
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Post by david23 » Thu May 17, 2007 4:32 pm

Clarification please, you mean ATPase right?

aceraf2612
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Post by aceraf2612 » Thu May 17, 2007 7:58 pm

AGPase which stands for ADP-Glucose Pyrophosphorylase

Im currently doing some experiment work which involves me placing leaf discs in glucose solution. From the results I have received starch is present in the leaf discs but what i dont understand is where does glucose join on with the glucose1-phosphate and where?

Cheers for the help

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