Yeast two hybrid

Genetics as it applies to evolution, molecular biology, and medical aspects.

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stone
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Yeast two hybrid

Post by stone » Tue May 15, 2007 5:54 pm

I understand how the fusion vector enters the yeast cytoplasm, but aren’t they needed to enter the nuclease in order to be express. How can we do that?

What in the case of the yeast 3 hybrid with the RNA fusion, how is that localized to the nuclease?

Thanks in advance

Marla

david23
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Post by david23 » Wed May 16, 2007 2:26 am

this is not yeast two hybrid, or maybe we are not talking about the same things. The hybrid proteins thing are there to assay whether two proteins of interest bind or not. But your question is more general, so first it's nucleus, and not nuclease. 2nd, it's the proteins that fuse after expression, not the vectors. Third, yeasts have mechanisms to express the genes on the vectors when it needs to.

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