gel electrophoresis / barr body

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novo'
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gel electrophoresis / barr body

Post by novo' » Sun May 13, 2007 10:31 am

hey there
i'm gonna take the Bio AP tomorrow morning and I've got 2 questions

1) What is gel electrophoresis and how can i solve proplems asked on this topic ?

2) Is the barr body the structure that deactivates one of the female X-chromosomes, or is it the actual inactive X-chromosome in females ?

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canalon
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Post by canalon » Mon May 14, 2007 6:02 pm

1) see that, it should answer most of your questions (plus links in it). And it was correct at the time I checked
Last edited by canalon on Wed Sep 26, 2007 2:15 am, edited 1 time in total.
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david23
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Post by david23 » Wed May 16, 2007 5:27 pm

the barr body has genes/proteins that differ from the other x chromosome. You can tell the difference by applying electrophoresis to assay a cell to see whether a protein of interest known to be on one x chromosome is present or not. Simple huh

muhammed shady
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Post by muhammed shady » Tue Sep 25, 2007 2:12 pm

Sorry .. I'm asked for a research from the Professor to know what is the practical method applied to identify the Barr Body ..
Is there somebody have the answer ?

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MrMistery
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Post by MrMistery » Wed Sep 26, 2007 5:23 pm

well, the barr body can be seen at an optical microscope after appropriate coloration. If you want to be sure, use FISH.
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