sterilization

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binay
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sterilization

Post by binay » Tue Apr 24, 2007 10:50 am

how do I sterilise saturated glycerol? I want to use it for stock culture. please advise.

blcr11
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Post by blcr11 » Tue Apr 24, 2007 11:56 am

You can either autoclave it for 10 min on liquid cycle, or dilute it to 50% v/v in water and filter sterilize it through 0.2 micron filtration unit.

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