Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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- Posts: 5
- Joined: Thu Apr 19, 2007 2:33 pm
what it is the quantity (in µl) of ethidium bromide to add in a electrophoresis gel for have DNA bands quite visible with UV (Ultrat violet)?
thanks for your helping
- Posts: 436
- Joined: Mon Mar 20, 2006 8:36 pm
I think I add about.... 6microliters of EtBR to each 100mL of agarose soln. (I add it when the agarose soln. is 'cool' enough to pour)
.. however, i do not know the concentration of the EtBR off hand.... but I find that a little goes a long way.
- Posts: 227
- Joined: Thu Aug 17, 2006 7:20 am
About 0.5 ug/ml final concentration in your gel should be OK.
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