hyperchromic effect

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biology_06er
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hyperchromic effect

Post by biology_06er » Wed Apr 18, 2007 1:17 pm

hi there

for our lab we had a sample of DNA and nucleotide...we measured their absorbance at 260 and 280. we then boiled them both for 5mins and measured their absorbance. DNA for both wavelengths increased but for N.T not very much (at 280 it decreased very slightly)...umm can someone tell me why...i know for the DNA its cos the bases are free to move in solution and hence they can absorb more UV light...but what about the N.T i don't really get that...and also I dont really get the point in measureing the absorbance with 2 diff. wavelengths--??

pleeease help me
biology_06er

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