acrylamide gels

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khan_awais
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acrylamide gels

Post by khan_awais » Tue Apr 17, 2007 7:53 pm

Hi,
If anyone help me regarding:
I want to run ssr on acrylamide gels, for denaturing acrylamide gel which stain is good/Can I use syber gold to stain (consider single stranded DNA, as it will be denatured and Ethidium bromide could not be used).
Thanks

blcr11
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Post by blcr11 » Wed Apr 18, 2007 3:53 pm

I would think the SYBR stains would work. From the product literature it looks reasonable. I've not had a chance to use any of them myself yet.

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canalon
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Post by canalon » Wed Apr 18, 2007 9:01 pm

WE use Sybr-gold for staining acrylamide gels in DGGE, and it works without problem. But it is for dsDNA. I never tried to detect ssDNA, so If you think know that Sybr-gold can detect it, acrylamide should not be a problem.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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