GraphPad Prism

Debate and discussion of any biological questions not pertaining to a particular topic.

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Dr.Stein
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GraphPad Prism

Post by Dr.Stein » Mon Apr 09, 2007 3:40 am

Anyone familiar with this biostatistic software? I want to have old version 3.0 , 4.0, or 4.3. Demo version will accepted. It can be used for 30 days :lol:

Thanks :D
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mith
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Post by mith » Mon Apr 09, 2007 3:58 am

My school recommends this freeware
http://www.r-project.org/
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Post by Dr.Stein » Mon Apr 09, 2007 5:11 am

Does it provide standard curve + interpolation menu and calculation for enzyme kinetics?
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mith
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Post by mith » Mon Apr 09, 2007 2:02 pm

I have no idea lol, but it does have multidimensional distance analysis so I think it's pretty advanced.
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Post by Dr.Stein » Tue Apr 10, 2007 9:31 am

I am stuck with my ELISA data analysis again :(
I read in its protocol that I need "Four Parameter Logistic" software to generate proper standard curve. Anyone knows how to find this software? Thank you.
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Post by Vagabond » Tue Apr 10, 2007 12:14 pm

I believe Excel will graph it for you. As for calculating it off the graph you need an equation. I will look around I know I used to have one in a file somewhere but Now I use Prism, StatView or SoftMax
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Post by CoffeaRobusta » Tue Apr 10, 2007 2:31 pm

What was wrong with this software that someone else told you to use?

http://www.cdc.gov/ncidod/dbmd/bimb/elisa.htm

I quote from the page:
ELISA for Windows is able to abstract the standard series, individual serum samples, and quality control samples from this file. The standards data are used to form a characteristic or standard curve which may be modeled using a three point cubic spline or a four parameter logistic-log function. The four parameters of the logistic function may be estimated using two methods: iteratively reweighted least squares and robust procedures. Estimation options include the Taylor series linearization (Gauss-Newton) and the Marquardt's compromise estimation algorithms. The standard curve is then used to interpolate antibody concentrations for the patient isolates and quality control samples. Summary statistics are calculated from these concentrations (means, standard deviations, coefficients of variation, etc). ELISA for Windows also forms plots of the standards data with the estimated standard curve superimposed on the data points.

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Post by fcs » Mon Jul 27, 2009 11:31 pm

I know this thread is out of date but I hope this information will reach others that are facing a similar question.

The 4-parameter logistic model equation assumes symmetry around the inflection point whereas the 5-parameter logistic model equation takes into account the asymmetry that is present in most bioassays including elisas.

Here is a blog post that goes into the 5-parameter logistic in more detail.


MasterPlex ReaderFit is specifically designed for drawing standard curves and curve-fitting of unknown points from elisa assays.

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Post by assaywiz » Sun Oct 23, 2011 10:26 pm

Or you could use the free tools at:

http://www.myassays.com/four-parameter-fit.assay
http://www.myassays.com/five-parameter-fit.assay

These are preconfigured and require no download or installation.

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