chromatography

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logisticslord2
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chromatography

Post by logisticslord2 » Wed Apr 04, 2007 5:56 pm

We did chromatography for nucleic acids. There are 3 Rf values calculated. They are .76, .44, and .21. The values given for RNA=.71 and DNA=.39. How do we explain all three of these then? Does it have to do with what bases are present? Help please...if you have a link that's excellent but if not that's cool too..

sdekivit
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Post by sdekivit » Sat Apr 07, 2007 10:02 am

it can be a slight mistake in you mobiule phase (slightly more polar, since the Rf-values are higher if you use normal phase).

Baed on your results, i would say Rf = 0,76 = DNA and Rf = 0,44 = RNA. Probably the other fraction is unremoved denatured protein.

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