effect of temperature on the survival of yeast cells

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Ulbrid
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Post by Ulbrid » Sat Apr 28, 2007 5:02 pm

dee88 wrote:im at work now but im sure il have some more questions when i begin to write it up be sure to come back on at 8 :D


Right, i'll be back on later.
I should really improve my plan because.... it really sucks. Have you started yours yet? I'm finding it really difficult to keep to the word limit; it's already 2000 words.

01addiv-llangirb
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Post by 01addiv-llangirb » Sat Apr 28, 2007 7:06 pm

ulbrid you put your finger on it all adding the methelyne blue then heationg does is make it harder to tell dead and alive apart under the microscope?
Physics more and more knowledge about less and less. - Still it's the basis for all else.

Ulbrid
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Post by Ulbrid » Sat Apr 28, 2007 7:43 pm

01addiv-llangirb wrote:ulbrid you put your finger on it all adding the methelyne blue then heationg does is make it harder to tell dead and alive apart under the microscope?


No, but i think it's just better to add it afterwards; there's no real reason for it really. Although i think i can remember my teacher me, when doing a prelim., to add the indicator in afterwards; she didn't give a reason for it, either.

01addiv-llangirb
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Post by 01addiv-llangirb » Sat Apr 28, 2007 7:48 pm

long shot and already covered it may be down to the decompistion process it can undergo when heated
Physics more and more knowledge about less and less. - Still it's the basis for all else.

monkeycraze_jo
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Post by monkeycraze_jo » Sat Apr 28, 2007 8:36 pm

Hey, anyone manage to get any sensible colorimeter readings? It would be super if you could share at least an idea or the range you got.
Cheers =)

monkeycraze_jo
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Post by monkeycraze_jo » Sat Apr 28, 2007 8:37 pm

Isn't quite funny how we're all using code names? I'm loving it hehehe

Ulbrid
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Post by Ulbrid » Sat Apr 28, 2007 8:57 pm

monkeycraze_jo wrote:Hey, anyone manage to get any sensible colorimeter readings? It would be super if you could share at least an idea or the range you got.
Cheers =)


I'm guessing it'd be hard to get any sort of reading because the yeast suspension, unless heavily diluted, is opaque so any change in blue intensity isn't going to make a difference.

Ulbrid
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Post by Ulbrid » Sat Apr 28, 2007 8:58 pm

monkeycraze_jo wrote:Isn't quite funny how we're all using code names? I'm loving it hehehe


Heh, yeah. I wonder if anyone's caught onto this, yet. It's not like anyone's cheating, anyway; i mean, we're free to browse the internet, aren't we?

monkeycraze_jo
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Post by monkeycraze_jo » Sat Apr 28, 2007 9:36 pm

Lol...I hope so =)

Anyone manage to make out any graphs then?

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Post by Ulbrid » Sat Apr 28, 2007 10:15 pm

monkeycraze_jo wrote:Anyone manage to make out any graphs then?


I didn't think we were needed to, being only a plan we're writing.

bioprince
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graph for the effect of temperature on the yeast cells

Post by bioprince » Sun Apr 29, 2007 8:31 am

i just wanted to know if it is alright to draw a sketch graph for the effect as i don't have any results. it is the bio OCR coursework. :?

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Post by bioprince » Sun Apr 29, 2007 9:01 am

the reason for adding afterwards is that the methylene blue can tehrmally decompose into different oxides then it won't work

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