DNA/RNA extraction

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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arm_chair27
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DNA/RNA extraction

Post by arm_chair27 » Mon Mar 26, 2007 12:57 pm

what's different between using 95%ethanol and isopropanol in DNA/RNA extraction?

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canalon
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Post by canalon » Mon Mar 26, 2007 4:20 pm

Isopropanol is slightly better at precipitating DNA (and butanol even better). It does need to be frozen or even a long incubation. And it might even be cheaper tha ethanol in many countries were alcohol is heavily taxed.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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