TBE

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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lumena
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TBE

Post by lumena » Mon Mar 19, 2007 7:19 pm

Hi

Can you save and reuse TBE (tris borate edta) after running an agarose gel :?:

what is your experience :?:

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canalon
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Post by canalon » Mon Mar 19, 2007 7:30 pm

Often reuse it without problems. IF you want publication grade picture, you might want to work with fresh reagents, otherwise I'd say 5 times without problem, and up to 10 if you are careful (top it with water after use to compensate for evaporation and electrolysis) and I still get gels that are good enough to read a PCR result.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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