Chloroplast DNA Isolation terminology

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Cha0s
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Chloroplast DNA Isolation terminology

Post by Cha0s » Fri Mar 16, 2007 5:48 pm

Hi everyone. I'm trying to use this protocol to isolate chloroplast DNA: http://wheat.pw.usda.gov/~lazo/methods/ ... plchl.html

I have three questions:
First, could I use this protocol with arabidopsis plants?
Second, what does "7 vol LL" mean in that first step? I've looked all over his site and I can't find anything on it. Thanks in advance for your help!
Third, what does "DIECA/L" mean (also in the first step)?

EDIT: typo!

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