RNA Gel

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Sheira
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RNA Gel

Post by Sheira » Sun Mar 11, 2007 11:01 am

Hi Guys,

I am doing in vitro transcription assay with Epicenter E. coli RNA polymerase. Every time I run the sequencing gel (standard 6M Urea in TBE) I got a smear all the way through. I can still see my transcript but the gel is really not publication quality. Any ideas please please help!!:)

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