immunfluorescence microscopy

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biology_06er
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immunfluorescence microscopy

Post by biology_06er » Sat Mar 03, 2007 11:57 pm

Hi there

Just wondering in our lab we did the other day using the above technique we added a secondary antibody to the primary antibody and then to the secondary we added the dye..but in my book it gives a description of this technique only using only one antibody...can someone tell me why in some cases a secondary antibody is also used?

Hope I make sense
Thanks
biology_06er

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canalon
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Post by canalon » Sun Mar 04, 2007 3:06 am

Signal amplification : more than one secondary antibody can detect the first one, so you have one fluororfore per epitope.
Patrick

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