N-Cadherin western blotting

[Irene76]

N-Cadherin western blotting

• Quote

Post by Irene76 » Sun Dec 09, 2007 11:16 am

Hi,
I need to perform a western blotting using a N-Cadherin antibody. I used the antibody from BD Bioscences on hela, huvec and human fibroblasts, hoping these cells were good positive controls but I had no results. Do You know if the matter is caused by bad antibody supplied from BD or bad cells as positive control?
Thanks

[blcr11]

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Post by blcr11 » Mon Dec 10, 2007 2:04 pm

I guess I'd blame the cells before I'd blame the kit, but I don't know enough about either to offer much advice. Make sure you're diluting the antibody and doing any blocking correctly--but I imagine you've checked over obvious things like that. Maybe try a cell line of neurological origin?

[Irene76]

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Post by Irene76 » Mon Dec 10, 2007 5:34 pm

Thank you for your advices, but unfortunatly I don't have a cell line of neurological origin because I work with normal human keratinocytes that I have infected with a gene responsable of EMT. The only cell lines that I have are the ones that I have tested.
Regards