acrylamide question

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benedictine
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acrylamide question

Post by benedictine » Thu Nov 15, 2007 2:18 pm

Dear friends
can someone please advise me how to remove acrylamide that has solidified (left in beaker left in refrigerator)?

thank you
from bene

blcr11
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Post by blcr11 » Thu Nov 15, 2007 2:41 pm

You can just break it into pieces and throw it in the wastebasket. Once it's polymerized, acrylamide is no longer much of a toxin.

benedictine
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glass

Post by benedictine » Fri Nov 16, 2007 3:48 pm

thank you for your reply
The problem is that it is inside a glass "bottle" like an erpenmeiyer (I dont know how it is called in english) but its glass with a round bottom and the top is very long. Kind of like a lab wine-bottle. I dont know the exact name.

that means we can never use this glass bottle thing anymore.
If the only way to get rid of the acrylamid is to break it, that means we throw away the whole thing?

thanks from bene

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Post by blcr11 » Fri Nov 16, 2007 4:26 pm

The simplest thing is to break it up with a spatula or plastic pipet until the pieces are small enough to fall through the mouth. Then you can just rinse out the little gritty things left behind. If the flask is so large a normal spatula or pipet won’t reach, then you’ve got a problem. You might try some acid chromate cleaning solution. Qiagen sells a kit for recovery of nucleic acids from gel fragments that actually dissolves the acrylamide. It’s designed for fairly small volumes of acrylamide, though. I always thought the active ingredient was a chaotropic salt like LiSO4 or LiCl, but I don’t know for sure. I guess you could try heating in 1N NaOH or 1N HCl to see if you can hydrolyze the amide links. Maybe KMnO4 cleaning solutions might work?

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canalon
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Post by canalon » Fri Nov 16, 2007 5:47 pm

Yeah I am not sure that using chaotropic ions in a large quantity is good idea, because you should not just get rid of them down the sink afterwards...
Another thing I would suggest is to just put something with sharp/pointy sides that could fit through the Erlenmeyer (yes the name is the same in English and french) mouth and bounce it inside until it breaks up the acrylamide.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

benedictine
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thanks

Post by benedictine » Mon Nov 26, 2007 10:59 pm

to canalon and blr11
thanks for your advice and sorry I am replying late
The acrylamide was in a glass round "bottle" with a long neck; this is the description so it cannot be reached by spatula or spoon.

Also, 2 days ago I couldn't find the bottle; I also dont know who removed it, or what happened to the bottle . So until now I have not tried what you suggested. I am letting you know what really happened.

thanks again
from bene

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