Growing one cell to many cell

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aaroncjj_2020
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Growing one cell to many cell

Post by aaroncjj_2020 » Fri Jun 08, 2007 8:46 am

Dear All,

I would like to know that if i have only one cell, wat is the method the enable me to get the karyotype of the chromosome?

as i know we can do it from blood extraction. however this time i only supply with only one cell.
Please help... thanks

blcr11
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Post by blcr11 » Fri Jun 08, 2007 1:32 pm

OK, growing many cells from one cell is not quite the same thing as trying to get a karyotype from a single cell. Which is it you want to do? If you are growing adherent cells like fibroblasts, just titrate them out at low density. Wait a few days and then isolate individual "colonies" of cells. Use a small cloning cylinder to isolate the colony from anything else on the plate, trypsinize within the cylinder and transfer the released cells to a fresh plate and grow that plate up and you've got a primary or secondary clone of cells that were derived from a single cell. This is the classic tissue-culturist way of cloning from the days before recombinant DNA. If your cells are non-adherent, you can do the same thing, but you have to titrate into small flasks at titers where you expect to transfer only one cell per volume transferred, but the principle is the same.

Again, this is not the same thing as getting a karyotype from a single cell.

aaroncjj_2020
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Growing one cell to many cell

Post by aaroncjj_2020 » Mon Jul 16, 2007 12:52 am

ok. if it is possible to get the karyotype from one cell, may i know the method and how it work? thanks a lot

blcr11
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Post by blcr11 » Wed Jul 18, 2007 3:06 pm

Posted: Fri Jun 08, 2007 10:18 am Post subject:

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I guess you can do it. I’m not that familiar with these things. Evidently there is even a patent out on it.

http://www.wipo.int/pctdb/en/wo.jsp?wo=2006091979

http://www.sigmaaldrich.com/sigma/gener ... ploidy.pdf

http://molehr.oxfordjournals.org/cgi/re ... 11/847.pdf

http://www.medscape.com/viewarticle/505220_6

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