synthetic enzyme substrates

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felicity_wishes
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synthetic enzyme substrates

Post by felicity_wishes » Wed Aug 23, 2006 6:23 am

Anyone know where I can get detailed information about synthetic substrates from? Something along the lines of their uses, purposes, history etc?

Any journals or websites would be really helpful.

Many thanks.
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weesper
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Post by weesper » Wed Aug 23, 2006 6:25 am

Give me an example and I'll try and help you
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felicity_wishes
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Post by felicity_wishes » Wed Aug 23, 2006 6:34 am

Well, anything from as broad as what they are and their purposes, to examples such as chromogenic or fluorogenic substrates for hydrolases/glycosidases.

Thank you.
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weesper
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Post by weesper » Wed Aug 23, 2006 7:22 am

Oh so you want to everything?

Go to http://www.syntheticsubstrates.com

Seriously if you're asking such as broad question I can only give you broad replies. Give me one concrete example.
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felicity_wishes
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Post by felicity_wishes » Wed Aug 23, 2006 7:35 am

Seriously - I need like a general overview. No details about their synthesis or one particular substrate or type of substrate. Just a general overview.

Perhaps something about their applications, and future prospects? Their use in clinical diagnosis?

PS. I don't know if you know, but that link doesn't work. Thanks for your help anyway.
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oppox
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Post by oppox » Wed Aug 23, 2006 11:07 pm

yea I think he knew :wink: . I think the best u can do is search it, search googles and in journals etc. I dont think u will find an overview in one place since its alot of substrates for alot of different proteins.
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weesper
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Post by weesper » Thu Aug 24, 2006 9:02 pm

Yes next time I'll also put a link up to http://www.canyouhandleajoke.com to make sure the message gets across. take care good luck
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felicity_wishes
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Post by felicity_wishes » Fri Aug 25, 2006 1:03 am

Thank you oppox for your reply :) I have tried google and journals, but can't really find much about where it all started and who came up with the idea and why - but I shall keep looking anyway! I need to write an introduction on "synthetic enzyme substrates and their applications". More specifically, I will need to focus on chromogenic substrates for glycosidases. Thanks again - I'll keep looking :)

Weesper - lol...I can handle a joke - I just didn't know it was one :)
So perhaps something like www.isitajoke?.com would be more uselful for me. I can click on the FAQ page and read up the "50 ways of determining when someone is being funny over the internet" section...
thanks anyway! :)
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canalon
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Post by canalon » Fri Aug 25, 2006 3:21 am

As far as I remember it started with Monod and the use of IPTG/Xgal to study the Beta-galactosidase activity. But it was long ago and I do not have the book anymore :(
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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felicity_wishes
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Post by felicity_wishes » Fri Aug 25, 2006 7:03 pm

Thank you for the information - it's helpful. I'll try searching that as well :)
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