Random priming method

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ak537
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Random priming method

Post by ak537 » Fri Oct 07, 2005 12:07 pm

Can any one write all possible(its 6 , I guess :roll: ) combinations of primers in random priming?

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canalon
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Re: Random priming method

Post by canalon » Fri Oct 07, 2005 1:06 pm

ak537 wrote:Can any one write all possible(its 6 , I guess :roll: ) combinations of primers in random priming?


No, Nobody will. Not because of the 'homework policy' of the forum, or because we don't want to hel you but because you made a little mistake.
Random priming is made with hexanucleotides, or 6 nucleotide long primers with random nucleotide at each position. Hence there isn't 6 combinations but 4^6 or 4096, and that is too much to type here :wink:

HTH

Patrick

ak537
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random priming

Post by ak537 » Sat Oct 08, 2005 12:06 pm

Thanks for this information. my concept is now clear about this and not even I wanna write all combinations :D :D

DevGrp
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Post by DevGrp » Wed Oct 12, 2005 2:15 pm

how about

NNNNNN

or occasionally

NNNNNNN

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