Microbiology question help

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seonguvai
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Microbiology question help

Post by seonguvai » Mon Apr 06, 2015 9:09 am

I need a basic layout on how to go about answering this type of question.

You have available an Hfr lac- ala+ vitB+ tetS strain of E. coli and an F- lac+ ala- vitB- tetS strain.

You also have a P1 transducing phage and a tube of plasmids with a gene for tetracycline resistance. These are the only supplies you can use ( besides media and reagents). Describe how you can isolate a pure strain of E . coli lac+ ala + vitB+ tetR, where all genes of interest are present in the chromosome of the bacterium. Include gene transfer steps you would use, as well as the media ( m9 or Lb) you would use to select for successful gene transfers

Would appreciate any help as soon as possible, thanks
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