Restriction Digesion

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Helics
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Restriction Digesion

Post by Helics » Sun Apr 06, 2014 4:07 pm

which is the best option for Restriction Endonuclease digesion ???

A) 37' C water bath
B) 37' C PCR program

MOmarKhan
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Post by MOmarKhan » Mon Apr 07, 2014 2:09 am

Both of them are okay! But i would go for Thermo-cycler instead of water bath.

Helics
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Post by Helics » Tue Apr 08, 2014 4:26 am

Dear scientist, Jack Bean I need your response.

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JackBean
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Post by JackBean » Sun May 04, 2014 12:04 pm

thanks for considering me a scientist :-D

I don't see much difference if you're sure that the temperature iscorrectly set. However, with thermocycler (or thermoblock) you do not have to worry, that you will spill your samples :)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Javedz2001
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Post by Javedz2001 » Sun Oct 11, 2015 5:19 pm

Water bath is NOT recommended because after time the sample will evaporate and stick on the top of the cap. This will change the buffer composition. Always use PCR machine with heated lid or dry incubator.

Javed Siddiqi

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