Please guide me about these restriction enzymes

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2angel
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Please guide me about these restriction enzymes

Post by 2angel » Tue Mar 11, 2014 7:24 am

For linirization of plasmid yeast(cervizea)why NotI is used instead of NHEI?
please compare and describe them

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JackBean
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Post by JackBean » Tue Mar 11, 2014 2:20 pm

http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

hematologym
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Post by hematologym » Mon Mar 17, 2014 11:17 am

you must select enzyme that cut your plasmid in one (or two) determined sequense and also not your target gene.

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